McGill Platform for
Cellular Pertubation
(MPCP)
The McGill Platform
for Cell Perturbation (MPCP)
provides all McGill researchers
with low-cost and rapid access
to the latest functional genetic
tools for biological research.
The MPCP was established in January 2017 and is part of the Rosalind and Morris Goodman Cancer Research Center (GCRC) and of the Biochemistry department. The mission of the MPCP is to support functional investigations of mammalian genomes by providing McGill researchers with complementary means of genetic perturbation that can be applied in order to understand gene function. These comprehensive technological platforms include arrayed and sequence-verified lentiviral genome-wide shRNA (gene suppression), cDNA (for gene overexpression) and CRISPR (for gene knockout/editing; human) libraries. These collections, in arrayed bacterial glycerol stocks, offer a renewable resource for diverse customized screening or testing of individual genes of interest across the human and mouse genomes.
It is typically very costly and time-consuming to obtain these reagents from commercial vendors. Our core service provides McGill researchers with rapid access to these essential genetic tools with at a minimal fee, greatly enhancing their research capacities.
Platform Director: Dr. Sidong Huang
Platform Manager: Dr. Azadeh Arabzadeh
Our Services
Main Instruments:
4x Ultra-Low Temperature Freezer, Panasonic model MDF-U76V
2x Infors HT Ecotron shaker incubator
1x VisionMate SR single rack 2D barcode reader
1x NanoDrop ND-2000C spectrophotometer
Placing the request online
Please use the following links for your clone search and service order: shRNA, CRISPR, or ORF. These are McGill campus-restricted websites. To access off-campus, please connect through VPN.
You can search either by Gene Symbol or by Gene ID. You can search for multiple genes at a time by using the “+” sign. You will see the service fee for your request once you select the clones and proceed to fill in the request form.
You need a FOAPAL or PO number to place your request.
Processing the request
Requests are processed once per week on Friday. Requests made after Thursday will be processed in the next cycle.
Once the request is processed, you will receive a confirmation email with instructions to pick up the bacterial cultures for your clones. The pickup date is usually the following Tuesday, or the week after depending on the number of requests.
The inoculation of shRNA, CRISPR, and ORF bacterial clones is carried out on Monday
*Picking up the clones
Please pick up your clone cultures during the hours indicated on the confirmation email. We are not responsible for problems caused by prolonged storage of the bacterial cultures if they are not picked up on time.
- Yang, X. et al. (2011). A public genome-scale lentiviral expression library of human ORFs. Nat Methods. Jun 26;8(8):659-61.
- The ORFeome Collaboration Nature Methods 13:191–192 (2016) doi:10.1038/nmeth.3776
- Erard, N. et al. (2017). A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout. Mol Cell. Jul 20;67(2):348-354.
List of genome-wide plasmid libraries:
MISSION® Human & Mouse lentiviral shRNA libraries (TRC1, 1.5 and 2)
MISSION® TRC3 Human ORF collection (cDNA)
MGC premier human lentiviral ORF, ORFeome Collaboration collection, ORFeome v8.1 Libraries
MGC premier mouse ORFeome Collaboration Library
transEDIT-dual CRISPR arrayed library, Human
If you have any question or special request, please contact us by filling the form below.
The facility, located in the Huang laboratory on the 8th floor of the McIntyre Medical building, includes a dedicated molecular biology wet lab and general office as well as shared access to a bacterial culture room and freezer farm space of the Biochemistry department.
Get in touch
We'd love to hear from you
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